Oncology

Team DDC

DNA Damage in Cancer

Often termed as a disease of the genome, cancer is the result of random acquisition of mutations that activate oncogenes and inactivate tumor suppressors. Consequently, cellular processes including cell cycle control, transcription, apoptosis and DNA repair are affected, conferring incremental growth advantages to cells and fomenting tumorigenesis. Advances in next-generation DNA sequencing have recently enabled the detailed characterization of molecular processes leading to such DNA alterations in cancer. It became immediately clear that alterations in DNA repair pathways could predispose cells to accumulating DNA damage. On the other hand, several drugs used in cancer treatment indeed target DNA repair pathways. Although this seems like a paradox in cancer treatment, it also spawns new avenues for therapeutical options through the use of synthetic lethality in cancer cells.

Our group is interested in extending the list of drugs that take advantage of the crosstalk between DNA repair genes and developing educated designs of targeted therapies for individual tumor types. We combine cancer genomics, genetic screening approaches and pre-clinical studies in order to dissect synthetic lethality mechanisms of DNA repair in human cells that can also act together with radio or chemotherapy. To this end, we perform detailed genetic and molecular characterization of DNA repair proteins based on tumor-specific somatic or germline alterations in cancer and apply CRISPR-Cas9 based genetic screens. Together, our research aims to understand the sensitivity or resistance of tumors to various treatment regimens and how inducing defects in DNA repair machinery can be used to selectively target tumors.

  • Mentors
    • Dr. Frank Zenke
      Head Cellular Pharmacology, Translational Innovation Platform Oncology at Merck (Industry mentor)
    • Dr. Amir Abdollahi
      Translational Radiation Oncology, German Cancer Research Center (DKFZ), Heidelberg (Academic mentor)
  • Publications
    1. Serçin Ö, Reither S, Roidos P, Ballin N, Palikyras S, Baginska A, Rein K, Llamazares M, Halavatyi A, Winter H, Muley T, Jurkowska RZ, Abdollahi A, Zenke FT, Neumann B, Mardin BR (2019):
      A solid-phase transfection platform for arrayed CRISPR screens
      Molecular Systems Biology 15(12)
    2. Drainas AP, Lambuta RA, Ivanova I, Serçin Ö, Sarropoulos I, Smith ML, Efthymiopoulos T, Raeder B, Stütz AM, Waszak SM, Mardin BR, Korbel JO (2020):
      Genome-wide Screens Implicate Loss of Cullin Ring Ligase 3 in Persistent Proliferation and Genome Instability in TP53-Deficient Cells
      Cell Reports 31(1)
The research of this team is kindly sponsored by Merck.

Our Team Members

Dr. Balca R. Mardin

Group Leader

  • Short Bio
    • 2012–2016: Postdoc with Dr. Jan O. Korbel, European Molecular Biology Laboratories (EMBL), Heidelberg, Germany
    • 2013–2015: Marie Curie IEF fellowship for postdoctoral work
    • 2008–2012: PhD with Prof. Elmar Schiebel (ZMBH), Ruprecht Karls University Heidelberg, Germany
    • 2005–2007: Master of Science in Molecular and Cellular Biology Program, Ruprecht-Karls-University, Heidelberg, Germany
  • Contact

Dr. Salvatore Benfatto

Postdoctoral Researcher

  • Short Bio
    • 2016-2018: Postdoc with Prof. Massimo Delledonne, Functional Genomics Lab, University of Verona, Italy
    • 2014: Visiting scientist at the Biocomputing Lab of Prof. Anna Tramontano, University “Sapienza” of Rome, Italy
    • 2013–2016: PhD with Prof. Giuseppe Teti, University of Messina, Italy
    • 2009-2012: Master of Science in Biology, University of Messina, Italy
  • Contact

Dr. Francesca R. Dejure

Postdoctoral Researcher

Amino acid transport in cancer

  • Short Bio
    • 2011-2017: PhD student with Prof. Dr. Martin Eilers, University of Würzburg, Germany, Research topic: Role of the transcription factor MYC as a metabolic-stress sensor in cancer
    • 2011-2014: Doctoral Scholarship of the Graduate School of Life Science (GSLS), University of Würzburg, Germany
    • 2008-2010: Master of Science in Medical Biotechnologies and Molecular Medicine, University of Bari, Italy
  • Contact

Dr. Özdemirhan Serçin

Postdoctoral Researcher

Targeted screens for investigating vulnerabilities of DNA repair deficiencies

  • Short Bio
    • 2012–2015: Postdoctoral research fellow in the laboratory of Prof. Dr. Cedric Blanpain, IRIBHM, Université Libre de Bruxelles, Belgium
    • 2006–2011: PhD Student and research assistant in the laboratory of Prof. Dr. Aziz Sancar, Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, NC, USA
  • Contact

Dr. Alexandra Vítor

Postdoctoral Researcher

Genetic screens to identify synthetic lethal mechanisms of DNA repair, as targets for cancer treatment

Nadja Ballin

Research Assistant

  • Short Bio
    • 2017-2018: Research Associate, Institute of Human Genetics, University Medical Center Freiburg, Germany
    • 2013-2016: Master of Science in Molecular Medicine, University of Freiburg, Germany
    • 2010-2013: Bachelor of Science in Molecular Medicine, University of Freiburg, Germany
  • Contact

Dr. Paris Roidos

Research Associate

Development of methods for tracing DNA repair status and identifying repair pathways

  • Short Bio
    • 2014–2016: Research assistant at Malformation genetics laboratory at Karolinska Institute, Stockholm and at the Musculoskeletal Research Centre at The University of Manchester, UK
    • 2012–2014: Master of Science Industrial Biotechnology, KTH Royal Institute of Technology, Sweden
    • 2006–2011: Bachelor of Science and Master of Science in Biological application, University of Ioannina, Greece
  • Contact

Kim Sigmund

Intern

  • Short Bio
    • Start September 2020: Master in Biomedical Science and Technology, University of Applied Sciences, Mannheim, Germany
    • 2019: Internship in the laboratory of Prof. Dr. Weinberg, Whitehead Institute for Biomedical Research as part of MIT, Cambridge, MA, USA
    • 2017-2020: Bachelor in Biotechnology, University of Applied Sciences, Mannheim, Germany
  • Contact
Image of

Close