“Single-cell RNA-sequencing made simple, efficient and inexpensive”, Dr. Simone Picelli, German Centre for Neurodegenerative Diseases (DZNE), Bonn, Germany

Speaker: Dr. Simone Picelli, German Centre for Neurodegenerative Diseases (DZNE), Bonn, Germany

Host: Postdoc Network DKFZ

  • Since 2017: Leader Operations and Development, Platform for Single Cell Genomics & Epigenomics (PRECISE) at the German Centre for Neurodegenerative Diseases (DZNE), Bonn, Germany

Outline of the talk

  • Single-cell RNA-sequencing (scRNA-seq)
  • Main divide between methods (5´- and 3´-tag vs full-length) highlighting their advantages and disadvantages
  • Smart-seq2 and other new full-length methods developed in the last 2 years
  • Generation of cDNA with Smart-seq2
  • From BD Rhapsody to Seq-well, from Drop-seq to the next version of Smart-seq2