“Proteome dynamics”, Prof. Dr. Matthias Selbach, Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany

Speaker: Prof. Dr. Matthias Selbach,Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany

Host: Dr. Balca R. Mardin, BioMed X

About Matthias:

  • since 2007: Junior Group leader at the Max Delbrück Center for Molecular Medicine (MDC)
  • 2004- 2007: Postdoctoral Fellow at University of Southern Denmark, Odense & MPI Martinsried

Outline of the talk

Understanding how the information in the genome is interpreted to produce a specific phenotype is probably the most important question in the post-genomic era. Proteins are the central link between the genome and the phenotype. Therefore, studying proteome dynamics can help to understand (i) how genomic information is processed to yield a specific proteome and (ii) how the proteins expressed at a certain condition affect the phenotype. Matthias will give two examples how mass spectrometry can be used to study proteome dynamics. First, he will present global pulse-chase experiments to quantify the kinetics of cellular protein degradation. These data show that many proteins are degraded non-exponentially. Many non-exponentially degraded proteins are members of multiprotein complexes which are produced in super-stoichiometric amounts. Importantly, this observation helps to explain the relationships between gene copy number and protein level changes. Second, Matthias will show how quantitative proteomics can help to understand the molecular mechanisms how disease-associated mutations cause disease. To this end, he will show the results of a proteomic screen designed to investigate how mutations in intrinsically disordered regions of proteins affect protein-protein interactions.